|
KMID : 1041220120500010015
|
|
Journal of Sericultural and Entomological Science
2012 Volume.50 No. 1 p.15 ~ p.19
|
|
|
The Possibility of Silk Protein to the Chondrogenesis
|
|
Jo You-Young
Kweon Hae-Yong
Lee Kwang-Gill
Lee Heui-Sam
Chon Jeong-Woo
|
|
|
Abstract
|
|
|
|
A number of researcher have studied biomaterials for cartilage regeneration and are now proceeding. Silk protein was attempted for use as biomedical materials by many researchers because it is natural polymer with biocompatibility and excellent mechanical strength. In this study, we want to know a possibility of silk protein on the cartilage regeneration. We isolated chondrocytes from nasal cartilage and confirmed optimal culture condition of the cells. To observe the effects of silk fibroin on chondrogenesis, we added silk fibroin solutions to the culture medium of chondrocyte and detected gene expression levels related chondrogenesis such as col2, col10. The chondrocytes showed optimal growth when they were cultured in DMEM medium supplemented with 10% FBS 100 ?IM ascorbic acid. The levels of col2 gene expression were increased in non-autoclaved silk fibroin, but decreased in autoclaved one. Also the gene expression levels of col10 were increased in silk fibroin, particulary at 3D culture. Based on the results of this study, we had seen the possibility of silk fibroin for cartilage regeneration. In future studies, we should know more clearly the relationship between cartilage regeneration and the silk protein.
|
|
|
KEYWORD
|
|
|
Silk protein, Chondrocyte, Cartilage
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|